Supplementary information

The file contains Supplementary Figures 1 - 4 with Legends, Supplementary Table 1 and additional references.

The Supplementary Figure 1 shows results confirming that SylA inhibits proteasomes irreversibly. The Supplementary Figure 2 shows that SylA inhibits bean proteasomes in crude extracts of etiolated bean seedlings. The Supplementary Figure 3 exhibits root tips of Arabidopsis seedlings homozygous for a CyclinB1;1-uidA (GUS) reporter fusion gene stained for GUS activity, showing that SylA treatment leads to enhanced staining of dividing cells. This indicates that the fusion gene product, which is expressed only in the G2/M transition of the cell cycle and then is degraded by the proteasome, accumulates upon SylA treatment. The Supplementary Figure 4 shows the results of in vivo proteasome inhibition assays in cultured human cells using the known proteasome inhibitor epoxomicin and the apoptosis-inducing agent cerulenin as positive and negative control agents, respectively. The Supplementary Table 1 lists statistical parameters of crystallographic data collection and refinement.

The file contains Supplementary Figures 1 - 4 with Legends, Supplementary Table 1 and additional references. - Download PDF file (484KB)