FIGURE 5. Structural basis of efficient metal exchange in CDCA1.
From the following article:
Structure and metal exchange in the cadmium carbonic anhydrase of marine diatoms
Yan Xu, Liang Feng, Philip D. Jeffrey, Yigong Shi & François M. M. Morel
Nature 452, 56-61(6 March 2008)
doi:10.1038/nature06636
a, Overlay of the structure of metal-free CDCA1-R1 (magenta) with that of the Cd-bound CDCA1-R1 (green). Details of the area indicated by the blue rectangle is shown in b. b, Comparison of the active site conformation between metal-free CDCA1-R1 (magenta) and the Cd-bound CDCA1-R1 (green). (A stereo view is shown in Supplementary Fig. 4a.) c, Effect of the mutation of Gly 316 and Gly 324 flanking the linkage sequence between two metal binding residues, His 315 and Cys 325, on Zn replacement for Cd in Cd-bound CDCA1-R2. Data shown for wild-type (open diamonds), G316A (green triangles) and G324A (blue squares). 12 
M Zn-PC2 complex was incubated with 10 M Cd-CDCA1-R2 and Cd-mutants in the presence of excess phytochelatin (PC2). Error bars (s.d.) represent duplicate measurements of a single experiment.