FIGURE 3. PGC-1
is induced by nutrient/oxygen deprivation and regulates VEGF independently of the HIF pathway.
From the following article:
HIF-independent regulation of VEGF and angiogenesis by the transcriptional coactivator PGC-1
Zoltan Arany, Shi-Yin Foo, Yanhong Ma, Jorge L. Ruas, Archana Bommi-Reddy, Geoffrey Girnun, Marcus Cooper, Dina Laznik, Jessica Chinsomboon, Shamina M. Rangwala, Kwan Hyuck Baek, Anthony Rosenzweig & Bruce M. Spiegelman
Nature 451, 1008-1012(21 February 2008)
doi:10.1038/nature06613
a, Primary skeletal muscle cells were infected with adenovirus expressing GFP (white bars) or PGC-1 (grey bars). After 48 h, HIF-1
mRNA (top), and protein (bottom) was measured. HIF-1 protein levels after treatment for 6 h with dipyridamole (dip.) or hypoxia (0.2% O2) are shown as controls. ct, control. b, ARNT- and ARNT+ cells were infected with adenovirus expressing GFP (white bars) or PGC-1 (grey bars), and VEGF mRNA was measured. c, C2C12 myotubes were treated with dimethyloxallyl glycine (DMOG) for 24 h, and expression of the indicated mRNAs was measured. d, ARNT– and ARNT+ cells were deprived of oxygen and nutrients for 3 h (grey bars), and expression of PGC-1 mRNA (top) and VEGF mRNA (bottom) was measured. Black bars, controls. Error bars indicate s.e.m.; n > 3 per group in all panels. Asterisk, P < 0.05 compared with control.
