FIGURE 2. Endogenous GABAA receptor signalling controls proliferation in ES and NCS cells.
From the following article:
Histone H2AX-dependent GABAA receptor regulation of stem cell proliferation
Michael Andäng, Jens Hjerling-Leffler, Annalena Moliner, T. Kalle Lundgren, Gonçalo Castelo-Branco, Evanthia Nanou, Ester Pozas, Vitezslav Bryja, Sophie Halliez, Hiroshi Nishimaru, Johannes Wilbertz, Ernest Arenas, Martin Koltzenburg, Patrick Charnay, Abdeljabbar El Manira, Carlos F. Ibañez & Patrik Ernfors
Nature 451, 460-464(24 January 2008)
doi:10.1038/nature06488
a, Immunofluorescence staining for vesicular GABA transporter (VGAT) in ES cells revealing a punctate localization (colour scale shows depth). b, Analysis of autocrine GABA signalling through the GABAAR. ES cell numbers with bicuculline for two passages (n = 3) are shown. c, BrdU pulse (2 h) of NCS cells treated with bicuculline for 12 h. d, Quantification of c (n = 74 clones). e, ES cell numbers four days after transfection with siRNA against the GABAAR 
3 subunit (n = 3). f, Photomicrograph of ES cell colonies in e. g, ES cell proliferation 48 h after siRNA transfection by the 2-h doublet assay (n = 3). h, BrdU incorporation over the course of 1.5 h in ES cells exposed to muscimol (n = 3). i, Quantitative analysis showing the percent change of cells in S-phase based on flow cytometric cell cycle profile analyses following muscimol or antagonist (picrotoxin) for 6 h by flow cytometry. j, Cell cycle profiles of i. PI, propidium iodide. Error bars indicate s.e.m. (two-tailed Student's t-test). Scale bars, 5 
m (a), 75 m (c) and 400 m (f).
