FIGURE 3. Fluridone blocked parasite growth by preventing egress.

a, Fluridone prevented parasite growth as monitored by -galactosidase activity in the parasite. b, Continuous culture in fluridone did not affect replication but inhibited egress from the vacuole. c, Treatment with exogenous ABA induced egress of parasites from mature vacuoles. Hours refers to length of culture before addition of ABA for 1 h and scoring of egress. Student's t-test; *P < 0.02, **P < 0.05. d, ABA reversed the inhibition of parasite egress caused by fluridone. Parasite-infected cells were treated with fluridone for 38 h, then stimulated with ABA for 1 h, and evaluated for egress. Student's t-test; *P < 0.002, **P < 0.05, ***P < 0.02. a–d, Data shown are means  s.e.m., N = 3 experiments.