Supplementary information
From the following article:
Toll-like receptor signalling in macrophages links the autophagy pathway to phagocytosis
Miguel A. Sanjuan, Christopher P. Dillon, Stephen W. G. Tait, Simon Moshiach, Frank Dorsey, Samuel Connell, Masaaki Komatsu, Keiji Tanaka, John L. Cleveland, Sebo Withoff & Douglas R. Green
Nature 450, 1253-1257(20 December 2007)
doi:10.1038/nature06421
Supplementary Information
This file contains Supplementary Methods and Supplementary Figures S1-S8 with Legends.
Supplementary Movie 1
This file contains Supplementary Movie 1 showing RAW cells expressing GFP-LC3 were fed with uncolored E. coli and confocal images were taken every minute. Representative frames of this movie are shown in Figure S3.
Supplementary Movie 2
This file contains Supplementary Movie 2 showing GFP-LC3 in RAW cells translocates to phagosomes containing zymosan (red). Representative frames of this movie are shown in Figure 1c.
Supplementary Movie 3
This file contains Supplementary Movie 3 showing RAW cells expressing GFP-LC3 were treated with chloroquine for 5 hours. Endoplasmatic reticulum was then stained with ER-Tracker (red). This movie depicts a full z-stack analysis of the macrophage shown in Figure S4b
Supplementary Movie 4
This file contains Supplementary Movie 4 showing RAW cells expressing GFP-LC3 were stained with ER-Tracker (red) and then fed with uncolored zymosan. Note that a small area in the center of the yeast is already red before uptake by the macrophage. A representative frame of this video is shown in Figure S4c.
Supplementary Movie 5
This file contains Supplementary Movie 5 showing RAW cells expressing GFP-LC3 and the PX-domain of p40 (phox) fused to mCherry (red) were fed with uncolored zymosan. The internalization of a yeast particle was followed at 1 min intervals. Representative frames of this movie are shown in Figure 3g.
Supplementary Movie 6
This file contains Supplementary Movie 6 showing GFP-LC3/PX-mCherry expressing macrophages were fed with uncolored zymosan and confocal images were taken every minute. Representative frames of this movie are shown in Figure S7.
Supplementary Movie 7
This file contains Supplementary Movie 7 showing GFP-LC3/PX-mCherry expressing macrophages were fed with uncolored beads and confocal images were taken every minute. Representative frames of this movie are shown in Figure S7.
Supplementary Movie 8
This file contains Supplementary Movie 8 showing Internalization of zymosan (red) was followed at 1.5 min. intervals in RAW cells transiently transfected with GFP-Beclin-1. Representative images of this video are shown in Figure 3h.
Supplementary Movie 9
This file contains Supplementary Movie 9 showing Phagocytosis of beads (uncolored) was followed at 1.5 min. intervals in RAW cells transiently transfected with GFP-Beclin-1.
Supplementary Movie 10
This file contains Supplementary Movie 10 showing GFP-LC3 expressing RAW cells were preloaded with lysotracker (red) and the phagocytosis of control beads was followed at 3 min. intervals. Representative frames of this video are shown in Figure 4a.
Supplementary Movie 11
This file contains Supplementary Movie 11 showing GFP-LC3 expressing RAW cells were preloaded with lysotracker (red) and the internalization of PAM3CSK4-covered beads was followed. Representative frames from this video are shown in Figure 4a.
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