Article

Nature 450, 1036-1042 (13 December 2007) | doi:10.1038/nature06418; Received 13 August 2007; Accepted 26 October 2007

The structural basis of calcium transport by the calcium pump

Claus Olesen1,2, Martin Picard3,4, Anne-Marie Lund Winther1,3, Claus Gyrup3, J. Preben Morth1,3, Claus Oxvig3, Jesper Vuust Møller1,2 & Poul Nissen1,3

  1. Centre for Membrane Pumps in Cells and Disease—PUMPKIN, Danish National Research Foundation, and,
  2. Institute of Physiology and Biophysics, University of Aarhus, Ole Worms Alle, blg. 1185, DK - 8000 Aarhus C, Denmark
  3. Department of Molecular Biology, University of Aarhus, Gustav Wieds Vej 10C, DK - 8000 Aarhus C, Denmark
  4. Present address: Laboratoire de Cristallographie et RMN biologiques, UMR 8015 CNRS, Faculté de Pharmacie. Université Paris Descartes, 4 avenue de l'Observatoire, 75270 Paris Cedex 06, France.

Correspondence to: Jesper Vuust Møller1,2Poul Nissen1,3 Correspondence and requests for materials should be addressed to P.N. (Email: pn@mb.au.dk) and J.V.M. (Email: jvm@biophys.au.dk).

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The sarcoplasmic reticulum Ca2+-ATPase, a P-type ATPase, has a critical role in muscle function and metabolism. Here we present functional studies and three new crystal structures of the rabbit skeletal muscle Ca2+-ATPase, representing the phosphoenzyme intermediates associated with Ca2+ binding, Ca2+ translocation and dephosphorylation, that are based on complexes with a functional ATP analogue, beryllium fluoride and aluminium fluoride, respectively. The structures complete the cycle of nucleotide binding and cation transport of Ca2+-ATPase. Phosphorylation of the enzyme triggers the onset of a conformational change that leads to the opening of a luminal exit pathway defined by the transmembrane segments M1 through M6, which represent the canonical membrane domain of P-type pumps. Ca2+ release is promoted by translocation of the M4 helix, exposing Glu 309, Glu 771 and Asn 796 to the lumen. The mechanism explains how P-type ATPases are able to form the steep electrochemical gradients required for key functions in eukaryotic cells.

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