Supplementary information
From the following article:
Engraftment of connexin 43-expressing cells prevents post-infarct arrhythmia
Wilhelm Roell, Thorsten Lewalter, Philipp Sasse, Yvonne N. Tallini, Bum-Rak Choi, Martin Breitbach, Robert Doran, Ulrich M. Becher, Seong-Min Hwang, Toktam Bostani, Julia von Maltzahn, Andreas Hofmann, Shaun Reining, Britta Eiberger, Bethann Gabris, Alexander Pfeifer, Armin Welz, Klaus Willecke, Guy Salama, Jan W. Schrickel, Michael I. Kotlikoff & Bernd K. Fleischmann
Nature 450, 819-824(6 December 2007)
doi:10.1038/nature06321
Supplementary Information
This file contains Supplementary Figures 1-4 with Legends, Supplementary Table 1 and Supplementary Methods with references
Supplementary Video 1
This file contains Supplementary Video 1 which shows in vivo monitoring of functional engraftment of GCaMP2+ embryonic cardiomyocytes in the infarct. Ca2+ signaling is detected within a section of infarcted left ventricle during sinus rhythm (heart rate: 490 bpm) in an anesthetized and ventilated mouse with open chest. Engrafted cells are coupled (2:1) to the native myocardium. GCaMP2 fluorescence only increases in case of coupling, the paradox movement of the infarcted area does not alter the fluorescence intensity. Recorded at 112 fps and displayed at 11.2 fps.
Supplementary Video 2
This file contains Supplementary Video 2 which shows optical voltage recordings in infarcted, sham injected mouse. Di-4-ANEPPS fluorescence signals during S1-S2 stimulation protocol in an isolated heart. The first 2 waves are produced by stimulations at 150 ms, followed by early activation at 80 ms cycle length (white dot indicates S2 start). Note the movement of the voltage wave around the infarct area and the induction of prominent circular waves by S2 stimulation (see also Fig. 4d). Recorded at 1 kHz and displayed at 66 fps.
Supplementary Video 3
This file contains Supplementary Video 3 which shows that engraftment of embryonic cardiomyocytes results in conduction of voltage wave into the infarct. Same stimulation protocol as in Supplementary Video 2. Note partial invasion of infarct by voltage wave during S2 stimulations and failure of S2 protocol (white dot) to produce boundary effects. Engraftment was confirmed by visualization of fluorescence of engrafted cells. Recorded at 1 kHz and displayed at 66 fps.
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