Supplementary information
From the following article:
Shiga toxin induces tubular membrane invaginations for its uptake into cells
Winfried Römer, Ludwig Berland, Valérie Chambon, Katharina Gaus, Barbara Windschiegl, Danièle Tenza, Mohamed R. E. Aly, Vincent Fraisier, Jean-Claude Florent, David Perrais, Christophe Lamaze, Graça Raposo, Claudia Steinem, Pierre Sens, Patricia Bassereau & Ludger Johannes
Nature 450, 670-675(29 November 2007)
doi:10.1038/nature05996
Supplementary Information
The file contains Supplementary Figures 1-14 with Legends, Legends for Supplementary Movies 1-11 and additional references.
Supplementary Movie 1
The file contains Supplementary Movie 1 which shows confocal live cell imaging of tubule growth after injection of 20 nM Alexa488-labeled STxB onto energy-depleted HeLa cells at 37°C.
Supplementary Movie 2
The file contains Supplementary Movie 2 which shows confocal live cell imaging of tubule growth at 37°C following binding of 20 nM Alexa488-labeled STxB onto energy-depleted HeLa cells at 4°C.
Supplementary Movie 3
The file contains Supplementary Movie 3 which shows confocal live cell imaging of tubule growth at 37°C following binding of 20 nM Alexa488-labeled STxB onto energy-depleted HeLa cells at 4°C.
Supplementary Movie 4
The file contains Supplementary Movie 4 which shows evanescent field live cell imaging of tubule scission at 37°C after wash out of energy poisons.
Supplementary Movie 5
The file contains Supplementary Movie 5 which shows confocal real time imaging experiment showing tubule formation on a GUV composed of DOPC/cholesterol/Gb3 after injection of Cy3-labeled STxB.
Supplementary Movie 6
The file contains Supplementary Movie 6 which shows confocal real time imaging showing tubule dynamics at the equatorial plane of a GUV composed of DOPC/cholesterol/Gb3 at steady state.
Supplementary Movie 7
The file contains Supplementary Movie 7 which shows spinning-disk confocal real time imaging experiment showing tubule dynamics at steady state in 3D-projection at the equatorial plane of a GUV composed of DOPC/cholesterol/Gb3 during incubation with Cy3-labeled STxB.
Supplementary Movie 8
The file contains Supplementary Movie 8 which shows confocal real time imaging experiment at the equatorial plane of a GUV composed of DOPC/cholesterol/Gb3 in the absence of STxB.
Supplementary Movie 9
The file contains Supplementary Movie 9 which shows confocal real time imaging experiment on a GUV composed of DOPC/cholesterol/Gb3 onto which the Cy3-labeled W34A mutant was injected.
Supplementary Movie 10
The file contains Supplementary Movie 10 which shows confocal real time imaging experiment on a GUV composed of DOPC/cholesterol and Gb3 with a single unsaturated C22:1 acyl chain.
Supplementary Movie 11
The file contains Supplementary Movie 11 which shows confocal real time imaging experiment on a GUV composed of DOPC/cholesterol and Gb3 with a saturated C22:0 acyl chain.
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