FIGURE 1. Determining the architecture of the NPC by integrating spatial restraints from proteomic data.
From the following article:
Determining the architectures of macromolecular assemblies
Frank Alber, Svetlana Dokudovskaya, Liesbeth M. Veenhoff, Wenzhu Zhang, Julia Kipper, Damien Devos, Adisetyantari Suprapto, Orit Karni-Schmidt, Rosemary Williams, Brian T. Chait, Michael P. Rout & Andrej Sali
Nature 450, 683-694(29 November 2007)
doi:10.1038/nature06404
First, structural data (red) are generated by various experiments (black). Second, the data are translated into spatial restraints. Third, an ensemble of structural solutions that satisfy the data are obtained by minimizing the violations of the spatial restraints, starting from many different random configurations. Fourth, the ensemble is clustered into distinct sets of structures on the basis of their similarities, and analysed in terms of protein positions, contacts and configuration.
