FIGURE 4. Association of Tim1 and Tim4 via exosomes.
From the following article:
Identification of Tim4 as a phosphatidylserine receptor
Masanori Miyanishi, Kazutoshi Tada, Masato Koike, Yasuo Uchiyama, Toshio Kitamura & Shigekazu Nagata
Nature 450, 435-439(15 November 2007)
doi:10.1038/nature06307
a, Ba/F3-Tim4 incubated with PE-annexin V, and analysed by flow cytometry. Staining profile of Ba/F3 in red. b, Electron micrographs of Ba/F3, Ba/F3-Tim1 or Ba/F3-Tim4, incubated with biotin-annexin V and stained with streptavidin-colloidal gold. Gold particles on exosome-like vesicles indicated by arrowheads. Scale bar, 0.3 
m. c, Ba/F3-Tim4 or Ba/F3-Tim1 incubated with Tim1-Fc or Tim4-Fc, stained with FITC-anti-IgG, and analysed by flow cytometry. Staining profiles of Ba/F3 in red. d, Ba/F3-Tim4 incubated with Tim4-Fc in the absence or presence of annexin V, stained with FITC-anti-IgG. e, Tim4-Fc-coated latex beads incubated with Tim1-Fc in the absence (green) or presence of 10-fold (blue) or 50-fold (brown) concentrated exosomes, stained with PE-anti-Tim1, and analysed by flow cytometry. Profile without Tim1-Fc in red dotted line.
