FIGURE 3. Binding to phosphatidylserine.
From the following article:
Identification of Tim4 as a phosphatidylserine receptor
Masanori Miyanishi, Kazutoshi Tada, Masato Koike, Yasuo Uchiyama, Toshio Kitamura & Shigekazu Nagata
Nature 450, 435-439(15 November 2007)
doi:10.1038/nature06307
a, Structure of Tim4, showing signal sequence (S), IgV-like (IgV), mucin-like (Mucin), transmembrane (TM) and cytoplasmic regions (Cyt). a.a., amino acid. Tim4-Fc carries the Tim4 extracellular region and the Fc region of human IgG. b, Ba/F3-Fas treated with Fas ligand for the indicated periods, stained with annexin V or Tim4-Fc. c, A filter spotted with phosphatidylcholine (PC), phosphatidylinositol (PI), phosphatidylethanolamine (PE) or phosphatidylserine (PS), incubated with Tim4-Fc followed by anti-IgG. d, Microtitre plates coated with indicated phospholipids incubated with Tim4-Fc, followed by anti-IgG. The assay was performed in triplicate. e, Lipid overlay assay performed as c with Tim1-Fc, Tim2-Fc and Tim3-Fc. f, Structures of the extracellular region of Tim1, Tim2 and Tim4, and their hybrids, fused to human IgG. g, Microtitre plates coated with PS incubated with increasing concentrations of Tim1-Fc, Tim2-Fc, Tim4-Fc, or their hybrids.
