FIGURE 2. Both classes of AWC neurons respond to odour removal.

a–d, G-CaMP calcium responses of AWC^ON (a, b) or AWC^OFF (c, d) after removal from different concentrations of isoamyl alcohol (IAA; a, c) or benzaldehyde (BZ; b, d). e, Removal of 2,3-pentanedione activates AWC^OFF, but not AWC^ON. f, AWC^ON calcium responses in animals removed from isoamyl alcohol after different times of exposure. g, h, Secondary responses in AWC^ON after isoamyl alcohol was removed at 10 s, added back at 20 s (g) or 40 s (h), and then removed again 20 s later. Traces represent averages of 7–30 individual recordings (Supplementary Fig. 2). Bar graphs, average percentage change during the 10 s after odour removal (highlighted in yellow). Error bars and the shaded region around the curves represent s.e.m. The colour key for bar graphs is the same as that for the corresponding response curve.