Supplementary information
From the following article:
Identification of stem cells in small intestine and colon by marker gene Lgr5
Nick Barker, Johan H. van Es, Jeroen Kuipers, Pekka Kujala, Maaike van den Born, Miranda Cozijnsen, Andrea Haegebarth, Jeroen Korving, Harry Begthel, Peter J. Peters & Hans Clevers
Nature 449, 1003-1007(25 October 2007)
doi:10.1038/nature06196
Supplementary Figures
The file contains Supplementary Figures 1-4 with Legends.
In Supplementary Figure 1 it is shown that GFP-positive CBC cells in the small intestine of LGR5-Ires-CreERT2 knock-in mice are frequently Ki67 positive, confirming that they are typically cycling cells.
The general strategy used to generate the LGR5-Ires-CreERT2 knock-in mice is outlined in Supplementary Figure 2.
In Supplementary Figure 3 it is demonstrated that CBC cells and +4 cells differ in their relative sensitivity to radiation-induced apoptosis, confirming that they are distinct cell types.
Lgr5-positive CBC cells permanantly marked by the genetic activation of a lacZ reporter gene generate a stream of blue progeny migrating from the crypts onto the villi. This phenomenon is readily visible as blue ribbons on the whole mount pictures in Supplementary Figure 4.
Supplementary Video
The file contains Supplementary Video 1. This movie shows a confocal 3D reconstruction of an Lgr5 GFP-positive crypt (inverted). Approximately six GFP-positive CBC cells are visible as wedge-shaped cells located at the base of the crypt. Nuclei are counterstained in red.
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