Supplementary information
From the following article:
Architectural and mechanistic insights into an EHD ATPase involved in membrane remodelling
Oliver Daumke, Richard Lundmark, Yvonne Vallis, Sascha Martens, P. Jonathan G. Butler & Harvey T. McMahon
Nature 449, 923-927(18 October 2007)
doi:10.1038/nature06173
Supplementary Information 1
This file contains Supplementary Figures 1-12 with Legends, Supplementary Table 1 with the data collection statistics and additional references.
Supplementary Information 2
The file contains Supplementary Video 1 which shows EHD2 wild-type was over-expressed in HeLa cells for 24 h and imaged by EPI-fluorescence for approximately 30 min. Some of the tubules and puncta are dynamic.
Supplementary Information 3
The file contains Supplementary Video 2 which shows cropped area of EHD2 WT (Video 1) at higher resolution.
Supplementary Information 4
The file contains Supplementary Video 3 which shows EHD2 T94A was over-expressed in HeLa cells for 24 h and imaged by EPI-fluorescence for approximately 30 min. There are only tubules, and these are mostly static. Cropped movies show a smaller area at higher resolution.
Supplementary Information 5
The file contains Supplementary Video 4 which shows cropped area of EHD2 T94A (Video 2) at higher resolution.
Supplementary Information 6
The file contains Supplementary Video 5 which shows EHD2 I157Q was over-expressed in HeLa cells for 24h and imaged by EPI-fluorescence for approximately 30min. No tubules can be found and the puncta are mostly motile.
Supplementary Information 7
The file contains Supplementary Video 6 which shows cropped area of EHD2 I157Q (Video 4) at higher resolution.
Supplementary Information 8
The file contains Supplementary Data with PDB coordinates of the proposed EHD2 oligomer. Four EHD2 dimers (in the absence of the EH domain) were aligned as described in Methods. All lipid interaction sites point towards the putative membrane interface. Molecules B and C which have been used for the initial alignment with GBP1 are related via a 2-fold axis and the nucleotides of these molecules are oriented in a head-to-head fashion.
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