FIGURE 1. Development of aggressive progenitor cell lymphomas on Pax5 loss in B lymphocytes.

a, Kaplan–Meyer survival analysis of mice of the indicated genotypes. The number (n) of mice analysed is shown. b, Enlarged lymph nodes (arrowheads) in a Cd19-cre Pax5^fl/– tumour mouse. c, Size comparison of lymph nodes from a Cd19-cre Pax5^fl/– tumour mouse (right) and a control littermate (left). d–g, Eosin–haematoxylin-stained sections of the lung (d), liver (e), kidney (f) and thymus (g) of a Cd19-cre Pax5^fl/– tumour mouse. The infiltration patterns of all tumour mice are shown in Supplementary Table 1. h, Flow cytometric analysis of lymph node cells of a Cd19-cre Pax5^fl/– tumour mouse and a control Cd19-cre Pax5^fl/+ littermate. Cells within the forward and side scatter (FSC/SSC) gates (blue) were analysed. The entire immunophenotype of Cd19-cre Pax5^fl/– tumour cells is summarized in Supplementary Table 2. i, PCR analysis of VhJ558–DJh, V–J and V1–J rearrangements in tumour-infiltrated lymph nodes of Cd19-cre Pax5^fl/– mice. Rearrangements to all Jh and J segments (numbered) were detected in splenic B cells. The detailed characterization of the different immunoglobulin gene rearrangements by cloning and sequencing is summarized in Supplementary Fig. 2c.