Supplementary information
From the following article:
Gap junction adhesion is necessary for radial migration in the neocortex
Laura A. B. Elias, Doris D. Wang & Arnold R. Kriegstein
Nature 448, 901-907(23 August 2007)
doi:10.1038/nature06063
Supplementary Figures
This file contains Supplementary Figures 1-14 with Legends
Supplementary Video 1a
This file contains Supplementary Video 1a with 3D rotation of area in crosshair of Figure 1i. Rotation shows expression of Cx26 puncta (red, relevant puncta circled) at the interface between a Vimentin+ radial fiber (green) and a
-III tubulin+ migrating neuron (blue)
Supplementary Video 1b
This file contains Supplementary Video 1b. 3D rotation in Supplementary Video 1a showing only Cx26 puncta (red, relevant puncta circled) and the
-III tubulin+ migrating neuron (blue) to highlight the localization of the puncta at the region of contact between the migrating neuron and the radial fiber (not shown)
Supplementary Video 2a
This file contains Supplementary Video 2a with 3D rotation of area in crosshair of Figure 1j. Rotation shows expression of Cx43 puncta (red, relevant puncta circled) at the interface between a Vimentin+ positive radial fiber (green) and the leading branch of a
-III tubulin+ migrating neuron (blue)
Supplementary Video 2b
This file contains Supplementary Video 2b. 3D rotation in Supplementary Video 2a showing only Cx43 puncta (red, relevant puncta circled) and the
-III tubulin+ migrating neuron (blue) to highlight the localization of the puncta at the region of contact between the leading branch of the migrating neuron and the radial fiber (not shown).
Supplementary Video 3
This file contains Supplementary Video 3 showing time-lapse of control migrating neurons (see Fig. 5a for description).
Supplementary Video 4
This file contains Supplementary Video 4 showing time-lapse of migrating neuron expressing Cx43-shRNA (see Fig. 5c for description).
Supplementary Video 5
This file contains Supplementary Video 5 time-lapse of migrating neuron expressing Cx26-shRNA (see Fig. 5c for description).
Supplementary Video 6
This file contains Supplementary Video 6 showing time-lapse of migrating neuron expressing Cx43T154A-EYFP and Tomato (see Fig. 5d for description).
Supplementary Video 7
This file contains Supplementary Video 7 showing time-lapse of migrating neuron expressing Cx26T135A-EYFP and Tomato (see Figure 5f for description).
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