FIGURE 3. Rescue of connexin-shRNA induced migration phenotype by wild-type connexin and connexin mutants that make adhesions but not channels.

a, Cx43CM (function: channel, adhesion, C terminus), Cx43CMT154A (function: adhesion, C terminus), or Cx43CMT154A 1–238 (function: adhesion) significantly increased the fraction of cells in the cortical plate compared to EYFP when co-expressed with Cx43-shRNA (***P < 0.0001, t-test; EYFP, n = 6 brains; Cx43CM, n = 4; Cx43CMT154A, n = 5; Cx43CMT154A 1–238, n = 6). Cx43CMC61S (function: hemichannel, C terminus) did not rescue the migration defect (EYFP versus Cx43CMC61S, t-test P = 0.4727; Cx43C61S, n = 4 brains). b, Cx26CM (function: channel, adhesion, C terminus), Cx26CMT135A (function: adhesion, C terminus), or Cx26CMT135A 1–216 (function: adhesion) significantly increased the fraction of cells in the cortical plate compared to EYFP when co-expressed with Cx26-shRNA (***P  0.0001, t-test; EYFP, n = 4 brains; Cx26CM, n = 5; Cx26CMT135A, n = 5; Cx26CMT135A, 1–216, n = 5). For 26-shRNA rescue, only caudal (caud.) sections (as described in Methods) were used, because the migration phenotype is stronger caudally (Supplementary Fig. 3). Values represent means  s.e.m.