Supplementary information

From the following article:

A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila

Georg Dietzl, Doris Chen, Frank Schnorrer, Kuan-Chung Su, Yulia Barinova, Michaela Fellner, Beate Gasser, Kaolin Kinsey, Silvia Oppel, Susanne Scheiblauer, Africa Couto, Vincent Marra, Krystyna Keleman & Barry J. Dickson

Nature 448, 151-156(12 July 2007)

doi:10.1038/nature05954

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Supplementary Table 1

This file contains Supplementary Table 1 with molecular details of all UAS-IR constructs, including predictions of ON- and OFF-target genes, CAN repeats, and specificity score (s19). Specific details of potential OFF-target genes, including the number of possible 19-mer matches for each UAS-IR construct and each OFF-target gene, are available at http://www.vdrc.at.

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Supplementary Table 2

This file contains Supplementary Table 2 with all established transgenic RNAi stocks, indicating the linkage of the UAS-IR transgene and its viability and fertility in homozygotes. All lines are in an isogenic w1118 background and have been verified to contain the indicated transgene by genomic PCR.

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Supplementary Table 3

This file contains Supplementary Table 3 with relative knock-down of target gene mRNA levels. Data are mean plusminus s.e.m (n = 2).

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Supplementary Table 4

This file contains Supplementary Table 4 with phenotypic scores (0 – 10) for each defect class, for each of the two assays performed with each line crossed to actin5C-GAL4.

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