Supplementary information

Supplementary Figures 1-4 with Legends and Supplementary Table 1

Supplementary Figure 1 shows RT-PCR analysis and in situ hybridization of TRPM8. The prevalence of neurons that normally would express TRPM8 in trigeminal ganglia has not changed in the TRPM8-/- littermates.
Supplementary Figure 2 shows properties of C and AM fibers assessed using the skin-nerve preparation. Basal firing rates of sensory nerve fibers from TRPM8-deficient mice were substantially reduced compared to wild type controls. However all other electrical and mechanical properties of these fibers were unaltered by TRPM8 disruption.
Supplementary Figure 3 shows mechanical and thermal (heat) thresholds for wild type and TRPM8-deficient littermates as determined by Von Frey filament and radiant heat behavioral paradigms, respectively. Similar responses were observed for both genotypes.
Supplementary Figure 4 demonstrates that TRPA1-deficient mice have normal cold sensitivity, irrespective of whether male or female mice (not in estrus) were examined.
Supplementary Table 1 shows that DRG and TG neurons from wild type or TRPM8-/- mice respond similarly to a variety of different chemical and thermal stimuli, irrespective of genotype.

Supplementary Figures 1-4 with Legends and Supplementary Table 1 - Download PDF file (482KB)