Supplementary information

From the following article:

The menthol receptor TRPM8 is the principal detector of environmental cold

Diana M. Bautista, Jan Siemens, Joshua M. Glazer, Pamela R. Tsuruda, Allan I. Basbaum, Cheryl L. Stucky, Sven-Eric Jordt & David Julius

Nature 448, 204-208(12 July 2007)

doi:10.1038/nature05910

BACK TO ARTICLE

Supplementary Figures 1-4 with Legends and Supplementary Table 1

Supplementary Figure 1 shows RT-PCR analysis and in situ hybridization of TRPM8. The prevalence of neurons that normally would express TRPM8 in trigeminal ganglia has not changed in the TRPM8-/- littermates.
Supplementary Figure 2 shows properties of C and AM fibers assessed using the skin-nerve preparation. Basal firing rates of sensory nerve fibers from TRPM8-deficient mice were substantially reduced compared to wild type controls. However all other electrical and mechanical properties of these fibers were unaltered by TRPM8 disruption.
Supplementary Figure 3 shows mechanical and thermal (heat) thresholds for wild type and TRPM8-deficient littermates as determined by Von Frey filament and radiant heat behavioral paradigms, respectively. Similar responses were observed for both genotypes.
Supplementary Figure 4 demonstrates that TRPA1-deficient mice have normal cold sensitivity, irrespective of whether male or female mice (not in estrus) were examined.
Supplementary Table 1 shows that DRG and TG neurons from wild type or TRPM8-/- mice respond similarly to a variety of different chemical and thermal stimuli, irrespective of genotype.

Supplementary Video

This file contains Supplementary Video 1. This movie shows a representative two-plate choice test in which wild type or TRPM8-deficient mice are examined for exploratory behavior with surface temperatures adjusted to 30°C and 20°C, as shown. It is evident that the TRPM8-deficient mouse exhibits no preference for the warmer side during the 30 sec exploratory period, whereas the wild type littermate spends most of its time at 30°C. Moreover, the wild type mouse shows clear aversion to the colder side.

BACK TO ARTICLE

DOWNLOAD BROWSER PLUGINS AND OTHER APPLICATIONS

Flash movies

Audio files

Chemical structures

Mathematica

Microarray

Compressed Stuff files

Compressed Zip files

Systems Biology Markup Language files (SBML)

Chemical Markup language files (CML)

BACK TO ARTICLE