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Nature 448, 191-195 (27 June 2007) | doi:10.1038/nature05950;
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Derivation of pluripotent epiblast stem cells from mammalian embryos
Although the first mouse embryonic stem (ES) cell lines were derived 25 years ago using feeder-layer-based blastocyst cultures, subsequent efforts to extend the approach to other mammals, including both laboratory and domestic species, have been relatively unsuccessful. The most notable exceptions were the derivation of non-human primate ES cell lines followed shortly thereafter by their derivation of human ES cells.
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