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Letter
Nature 447, 875-878 (14 July 2007) | doi:10.1038/nature05878; Received 7 November 2006; Accepted 26 April 2007; Published online 16 May 2007
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Gastroenterologist
- Wayne State University
- Detroit, Michigan, USA
Research Fellow
- Beth Israel Deaconess Medical Center
- Beth Israel Deaconess Medical Center, 330 Brookline Avenue, Boston, MA 02215
Drosophila miR2 induces pseudo-polysomes and inhibits translation initiation
Rolf Thermann1 & Matthias W. Hentze1
- European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
Correspondence to: Matthias W. Hentze1 Correspondence and requests for materials should be addressed to M.W.H. (Email: hentze@embl.de).
Abstract
MicroRNAs (miRs) inhibit protein synthesis by mechanisms that are as yet unresolved1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11. We developed a cell-free system from Drosophila melanogaster embryos that faithfully recapitulates miR2-mediated translational control by means of the 3' untranslated region of the D. melanogaster reaper messenger RNA. Here we show that miR2 inhibits translation initiation without affecting mRNA stability. Surprisingly, miR2 induces the formation of dense (heavier than 80S) miRNPs ('pseudo-polysomes') even when polyribosome formation and 60S ribosomal subunit joining are blocked. An mRNA bearing an ApppG instead of an m7GpppG cap structure escapes the miR2-mediated translational block. These results directly show the inhibition of m7GpppG cap-mediated translation initiation as the mechanism of miR2 function, and uncover pseudo-polysomal messenger ribonucleoprotein assemblies that may help to explain earlier findings.
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