In response to questions raised recently about flow cytometry data reported in Fig. 1 of this Article, we had the flow cytometry data for Fig. 1 and the experimental approach used to generate them reviewed by flow cytometry experts.
The flow cytometry data in the original Fig. 1b were found to be flawed in that corresponding IgG isotype control tracings for several of the plots differ by 1 log in fluorescence intensity, even though the same IgG subtype was used. The plots for these antigens (CD19, CD34, Sca-1, Thy-1 and MHC II) in the original Fig. 1b should therefore not be relied upon as an accurate representation of MAPC surface marker profiles. Details of the specific flaws are individually listed as Supplementary Information to this Corrigendum. A corrected version of Fig. 1b is now provided as Fig. 1 below.
The FACS plots in the original Fig. 1b were obtained from the ROSA26 cell line also used for the blastocyst injection studies and the postnatal transplantation in NOD-SCID mice. In Fig. 1 we now provide additional FACS analysis data for these specific antigens from the GFP transduced mouse MAPC line we described in the original Article at population doubling 120 (this line was also used to demonstrate the single-cell origin of endothelium-like, hepatocyte-like and neuroectoderm-like cell differentiation in vitro). These data appear not to be subject to the same technical problem. On the basis of this analysis, we have summarized the FACS phenotype of MAPC in Table 1 below. The phenotype of MAPC isolated subsequently and published by our group1,2 are also not affected by the same flaws in the plots published in the original Article. It should also be noted that in the original Fig. 1b, the plot for MHC I was a duplication of the FACS plot for Mac-1, although the correct FACS plot acquisition data for MHC I was presented in the original Supplementary Fig. 1 of the original Article.
Although problems with these specific FACS plots (as published in Fig. 1) undermine their utility as markers of the MAPC surface phenotype, the specific errors in these FACS plots do not alter the conclusions of the Article. Nevertheless, we wish to inform other scientists of the problems with these published FACS profiles, and provide an accurate representation of the phenotype of MAPC we described.
We submitted some minor corrections to Nature in 2002, but owing to administrative errors by the authors and Nature these were not seen through to publication. The three changes requested are as follows.
(1) In the legend to Fig. 1b, the superscripts ‘k’ in MHC II (I-Ak) and MHC I (H- 2Kk) should be ‘b’.
(2) In the Methods under ‘Differentiation culture and analysis’, the concentration of 109 M dexamethasone should be 0.05 μM.
(3) In the Supplementary Information, the sequence of the primers rex-1 and oct-4 should correctly read as follows. Mrexa: aag cgt ttc ctg gat ttc; Mrexb: ttt gcg tgg gtt agg atg tg; Moct4a: gaa gcc gac aac aat gag aac; Moct4b: aca gaa cca tac tcg aac cac a.
References
Serafini, M. et al. Long-term lymphohematopoietic reconstitution from nonhematopoietic cells. J. Exp. Med. 129–139, 129–139 (2007)
Breyer, A. et al. Multipotent adult progenitor cell (MAPC) isolation and culture procedures. Exp. Hematol. 34, 1596–1601 (2006)
Additional information
The online version of the original article can be found at 10.1038/nature00870
Supplementary information
Supplementary Information 1
This file contains supplementary information. (PDF 13 kb)
Rights and permissions
About this article
Cite this article
Jiang, Y., Jahagirdar, B., Reinhardt, R. et al. Erratum: Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 447, 880–881 (2007). https://doi.org/10.1038/nature05812
Issue Date:
DOI: https://doi.org/10.1038/nature05812
This article is cited by
-
Hyaluronic Acid (HA) Scaffolds and Multipotent Stromal Cells (MSCs) in Regenerative Medicine
Stem Cell Reviews and Reports (2016)
-
The culture and differentiation of amniotic stem cells using a microfluidic system
Biomedical Microdevices (2009)
-
A microfluidic device for separation of amniotic fluid mesenchymal stem cells utilizing louver-array structures
Biomedical Microdevices (2009)
-
Long-term culture of a cell population from Siberian sturgeon (Acipenser baerii) head kidney
Fish Physiology and Biochemistry (2008)
-
Flawed data in multipotent cell study
Nature Reports Stem Cells (2007)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.