FIGURE 3. Overview of RACE experiments showing a gene fusion.

Transcripts emanating from the region between the DONSON and ATP5O genes. A 330-kb interval of human chromosome 21 (within ENm005) is shown, which contains four annotated genes: DONSON, CRYZL1, ITSN1 and ATP5O. The 5' RACE products generated from small intestine RNA and detected by tiling-array analyses (RxFrags) are shown along the top. Along the bottom is shown the placement of a cloned and sequenced RT–PCR product that has two exons from the DONSON gene followed by three exons from the ATP5O gene; these sequences are separated by a 300 kb intron in the genome. A PET tag shows the termini of a transcript consistent with this RT–PCR product.