FIGURE 3. Depletion of eif-6 disrupts regulation of protein levels of lin-4 miRNA endogenous targets.
From the following article:
MicroRNA silencing through RISC recruitment of eIF6
Thimmaiah P. Chendrimada, Kenneth J. Finn, Xinjun Ji, David Baillat, Richard I. Gregory, Stephen A. Liebhaber, Amy E. Pasquinelli & Ramin Shiekhattar
Nature 447, 823-828(14 July 2007)
doi:10.1038/nature05841
a, Schematic representation of lin-4 base-pairing in the 3' UTRs of lin-14 and lin-28 mRNAs22, 23. The breakpoint in lin-14(n355gf) that removes the downstream predicted lin-4 complementary sites is indicated. b, Protein was isolated from wild-type or lin-4(e912lf) nematodes fed vector control (-) or eif-6 (+) dsRNA-expressing bacteria at time points before (4 h) and after lin-4 miRNA (20 h, 30 h) expression, as indicated. Samples were analysed by western blotting to determine the LIN-14, LIN-28, EIF-6 and control actin and tubulin levels. c, Protein was isolated from lin-14(n355gf) nematodes as described in b and compared to lin-4(e912lf) samples at the 4- and 20-h time points.
