Supplementary information

Supplementary Information

This file contains Supplementary Methods, Supplementary Tables 1-3, Supplementary Figures 1-8 with Legends, Supplementary Notes and additional references. Supplementary Methods section describes materials and procedures. The Supplementary Tables contain a list of the identified HopU1 substrates (Supplementary Table 1), bacterial strains and plasmids used in this study (Supplementary Table 2), and the nucleotide sequences of the primers used in this study. The Supplementary Figures show that HopU1 is injected into plant cells by the type III secretion system of P. syringae and that a hopU1 mutant is reduced in virulence (Supplementary Fig. 1); that HopU1 suppresses ion leakage in plants (Supplementary Fig. 2); that HopU1-expressing plants are altered in the AvrRpt2-dependent HR (Supplementary Fig. 3); representative two-dimensional PAGE gels that led to the identification of HopU1 substrates (Supplementary Fig. 4); localization of HopU1 and HopU1 substrates in plant cells (Supplementary Fig. 5); data showing that an A. thaliana Col-0 knock-out mutant is homozygous for the T-DNA insert in the AtGRP7 locus (Supplementary Fig. 6); Similar data for an independent Atgrp7 mutant and pathogenicity-related phenotypes (Supplementary Fig. 7); and a proposed model of suppression of plant innate immunity by HopU1.

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