Supplementary information
From the following article:
Multimodal fast optical interrogation of neural circuitry
Feng Zhang, Li-Ping Wang, Martin Brauner, Jana F. Liewald, Kenneth Kay, Natalie Watzke, Phillip G. Wood, Ernst Bamberg, Georg Nagel, Alexander Gottschalk & Karl Deisseroth
Nature 446, 633-639(5 April 2007)
doi:10.1038/nature05744
Supplementary Figures and Table
This file contains Supplementary Figures S1-S3 with Legends and Supplementary Table 1.
Supplementary Movie 1
This file contains Supplementary Movie 1 which shows swimming of a transgenic C. elegans expressing NpHR (transgene zxEx29) in muscles is instantaneously, and repeatedly, inhibited by photoactivation of HR (duration of illumination is indicated by appearance of a yellow dot).
Supplementary Movie 2
This file contains Supplementary Movie 2 which shows swimming of a transgenic C. elegans expressing NpHR in cholinergic motoneurons (transgene zxEx33) is instantaneously inhibited by photoactivation of NpHR (duration of illumination is indicated by appearance of a yellow dot).
Supplementary Movie 3
This file contains Supplementary Movie 3 which shows transgenic C. elegans expressing NpHR-ECFP in muscles (transgene zxEx30). Movement is rapidly inhibited (3x) by photoactivation of HR, and the body relaxes and dilates. Duration of illumination episodes is indicated by appearance of a yellow dot.
Supplementary Movie 4
This file contains Supplementary Movie 4 which shows one transgenic C. elegans expressing NpHR in muscles (transgene zxEx29), and one non-transgenic control animal. Movement of the transgenic animal (on the left) is rapidly inhibited by photoactivation of HR (illumination indicated by a yellow dot), while the non-transgenic animal does not respond. FILE: suppl movie 5.wmv
Supplementary Movie 5
This file contains Supplementary Movie 5 which shows transgenic C. elegans expressing NpHR in cholinergic motoneurons (transgene zxEx33). Movement is rapidly inhibited by photoactivation of HR, and the body relaxes and dilates. Duration of illumination is indicated by appearance of a yellow dot.
Supplementary Movie 6
This file contains Supplementary Movie 6 which shows co-expression and -activation of ChR2(H134R)-EYFP and NpHR in cholinergic motoneurons of transgenic C. elegans (transgene zxEx34). The animal is illuminated with blue light (for ChR2 activation, indicated by a cyan dot), causing contractions, then, while ChR2 is still photoactivated, NpHR is photoactivated by yellow light (yellow dot), causing significant body relaxation. When NpHR activation ends, the animal contracts again (ChR2 still activated), and finally, when ChR2 activation ends, the animal relaxes to the initial body length.
Supplementary Movie 7
This file contains Supplementary Movie 7 which shows co-expression and rapidly alternating activation of ChR2(H134R)-EYFP and NpHR in muscles of transgenic C. elegans (transgene zxEx32). The animal is illuminated with alternating blue light (for ChR2 activation, indicated by a cyan dot), causing contractions, and yellow light (for NpHR activation, indicated by a yellow dot), causing significant body relaxation.
Supplementary Video Streaming
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