FIGURE 3. NpHR mediates tunable neuronal inhibition over a range of timescales.

a, Increasing intensities of light lead to stronger inhibition of spikes evoked by 200 pA of current injection (21.7 mW mm^-2 at 100% intensity). b, Per cent of spikes inhibited at three different light intensities (1 s of light exposure, mean  s.d.; n  =  7). c, Duration of effective inhibition as measured by the delay from light onset to the first escaped spike (mean  s.d.; n  =  7). d, Ten min continuous illumination (two successive sweeps) robustly inhibited neuronal spike trains elicited by current pulse injection (300 pA, 5 Hz). Dashed boxes show the inhibition on an expanded timescale. Per cent efficacy of inhibition over 10 min measured by breaking down the 10 min interval into 1 min bins (inset, mean  s.d.; n  =  5).