Nature 446, 672-675 (5 April 2007) | doi:10.1038/nature05633; Received 5 October 2006; Accepted 22 January 2007; Published online 18 March 2007

Hagfish embryology with reference to the evolution of the neural crest

Kinya G. Ota1, Shigehiro Kuraku1 & Shigeru Kuratani1

  1. Laboratory for Evolutionary Morphology, Center for Developmental Biology, RIKEN, Kobe 650-0047, Japan

Correspondence to: Shigeru Kuratani1 Correspondence and requests for materials should be addressed to S. Kuratani (Email: saizo@cdb.riken.jp).

Hagfish, which lack both jaws and vertebrae, have long been the subject of intense interest owing to their position at a crucial point in the evolutionary transition to a truly vertebrate body plan1, 2, 3, 4. However, unlike the comparatively well characterized vertebrate agnathan lamprey, little is known about hagfish development. The inability to analyse hagfish at early embryonic stages has frustrated attempts to resolve questions with important phylogenetic implications, including fundamental ones relating to the emergence of the neural crest1, 5, 6. Here we report the obtainment of multiple pharyngula-stage embryos of the hagfish species Eptatretus burgeri and our preliminary analyses of their early development. We present histological evidence of putative neural crest cells, which appear as delaminated cells that migrate along pathways corresponding to neural crest cells in fish and amphibians2, 7, 8, 9, 10, 11. Molecular cloning studies further revealed the expression of several regulatory genes, including cognates of Pax6, Pax3/7, SoxEa and Sox9, suggesting that the hagfish neural crest is specified by molecular mechanisms that are general to vertebrates. We propose that the neural crest emerged as a population of de-epithelialized migratory cells in a common vertebrate ancestor, and suggest that the possibility of classical and molecular embryology in hagfish opens up new approaches to clarifying the evolutionary history of vertebrates.


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