FIGURE 3. Foxp3-amplified gene expression constitutes a defining characteristic of thymic and peripheral T_r cells.

a, b, Genes differentially expressed in CD25⁺Foxp3^-CD4⁺ (T₂₅), T_r and T_fn cells relative to T_n cells were identified for both thymic (a) and peripheral (b) T-cell subsets. For each cluster, values and line thicknesses represent the number of upregulated or downregulated genes, and error bars represent the full range of log₂ expression ratios. c, Expression patterns of genes that may have a role, or have been demonstrated to have a role, in T_r cell effector function. Foxp3-mediated repression of genes encoding pro-inflammatory cytokines and chemokines is also shown.