FIGURE 4. T_r cells converted into T_h2 effector cells owing to decreased Foxp3 expression.

a, b, The production of IL-2, IL-4, IL-17 and IFN- in RFP⁺ CD4⁺ T cells sorted from FIR/+ mice and in GFP⁺ CD4⁺ T cells sorted from FILIG/+ mice (a), or in RFP⁺ and RFP^- CD4⁺ T cells from FIR/Y mice and GFP⁺ and GFP^- CD4⁺ T cells from FILIG/Y mice (b) is shown. c, Relative mRNA levels of Ifng, Lta (lymphotoxin A), Ltb (lymphotoxin B), Il4, Il13, Il10, Il2 and Il17 among RFP⁺, RFP^-, GFP⁺ and GFP^– CD4⁺ T cells isolated from male FIR/Y and FILIG/Y mice are shown. Data are mean  s.d. from two experiments. d, At the end of the experiments described in Fig. 3c, The expression of IL-4 and IFN- was examined in RFP⁺FIR/+ and GFP⁺FILIG/+ cells that were recovered from recipient mice. e, At the end of the experiments described in Fig. 2c (right panel), the expression of IL-4, IL-17 and IFN- was determined in co-existing RFP⁺FIR/+ cells and GFP⁺FILIG/+ cells as well as in RFP^-GFP^-FIR/FIR cells. All results are representative of at least three experiments unless stated otherwise.