FIGURE 1. Generation of the p53-LSL allele.
From the following article:
Restoration of p53 function leads to tumour regression in vivo
Andrea Ventura, David G. Kirsch, Margaret E. McLaughlin, David A. Tuveson, Jan Grimm, Laura Lintault, Jamie Newman, Elizabeth E. Reczek, Ralph Weissleder & Tyler Jacks
Nature 445, 661-665(8 February 2007)
doi:10.1038/nature05541
a, The p53-LSL allele was generated while engineering a point-mutant p53 allele31 when homologous recombination occurred between the LSL cassette and the point mutation (asterisk). b, Genotyping of p53-LSL mice by PCR (polymerase chain reaction) amplification of tail DNA. c, Western blot of passage 5 MEFs infected with empty or Cre-expressing recombinant adenoviruses (Adeno-empty and Adeno-Cre). d, Anti-p53 immunostaining of p53LSL/LSL (that is, p53-LSL) MEFs infected with Adeno-LacZ or Adeno-Cre and then treated with doxorubicin (0.2 
g ml-1; 13 h). DAPI, 4,6-diamidino-2-phenylindole. e, 3T3 protocol on p53LSL/LSL, p53-/- and wild-type (WT) MEFs. f, DNA content of early (P3) and late (P9) passage p53LSL/LSL or p53-/- MEFs. Diploid (2n) and tetraploid (4n) DNA content is indicated.
