FIGURE 2. Dll4-mediated Notch signalling regulates endothelial cell differentiation.
From the following article:
Inhibition of Dll4 signalling inhibits tumour growth by deregulating angiogenesis
John Ridgway, Gu Zhang, Yan Wu, Scott Stawicki, Wei-Ching Liang, Yvan Chanthery, Joe Kowalski, Ryan J. Watts, Christopher Callahan, Ian Kasman, Mallika Singh, May Chien, Christine Tan, Jo-Anne S. Hongo, Fred de Sauvage, Greg Plowman & Minhong Yan
Nature 444, 1083-1087(21 December 2006)
doi:10.1038/nature05313
a, The lumen-like structures (white arrows) formed by HUVECs growing in fibrin gels were absent in the presence of anti-Dll4 antibodies or DBZ. Instead, the sprouts were highly packed with cells (black arrows). The boxed area is shown enlarged in the bottom micrograph. b, Anti-Dll4 antibodies block arterial development. Confocal images of neonatal mouse retinas stained with 
-smooth muscle actin (ASMA; red) and isolectin (green). c, Confocal images of adult mouse retinas stained with ASMA and isolectin. Eight-week-old mice were treated with PBS or anti-Dll4 antibodies (10 mg kg–1, twice weekly) for two weeks.
