FIGURE 1. MDMX amplification correlates with decreased activity of the p53 pathway.
From the following article:
Inactivation of the p53 pathway in retinoblastoma
Nikia A. Laurie, Stacy L. Donovan, Chie-Schin Shih, Jiakun Zhang, Nicholas Mills, Christine Fuller, Amina Teunisse, Suzanne Lam, Yolande Ramos, Adithi Mohan, Dianna Johnson, Matthew Wilson, Carlos Rodriguez-Galindo, Micaela Quarto, Sarah Francoz, Susan M. Mendrysa, R. Kiplin Guy, Jean-Christophe Marine, Aart G. Jochemsen & Michael A. Dyer
Nature 444, 61-66(2 November 2006)
doi:10.1038/nature05194
a, b, Real-time RT–PCR analysis of p14ARF and MDMX expression was done on normal human fetal retinae at four stages of development and on seven retinoblastomas. Data from duplicate samples were normalized to GAPDH expression and are plotted as fold changes relative to that of FW10 human retinae (1.0-fold). Error bars represent the s.d. of two experiments. c, Samples analysed in a and b were immunoblotted for MDMX and 
-actin. d–g, FISH analysis for MDMX was done on 49 primary untreated retinoblastomas; tonsil was used as a normal diploid control. Blue fluorescence is the nuclear counterstain; green fluorescence is the internal chromosomal control; red fluorescence is MDMX. h–k, The same 49 retinoblastomas were immunostained for MDMX, MDM2, p53 and p21. A retinoblastoma sample lacking MDMX was used as a negative control (i). Scale bars, 10 
m.
