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Nature 443, 1017-1021 (26 October 2006) | doi:10.1038/4431017a; Published online 25 October 2006

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Michael Eisenstein1

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Light microscopy is undergoing a renaissance, with a huge range of tools and techniques for gathering biological data with unprecedented speed and resolution. Michael Eisenstein takes a closer look.

Confocal microscopy is now a well-established technique for the three-dimensional imaging of cellular structures. But despite its success, the technique has its limitations when imaging live cells.