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Letter

Nature 443, 355-358 (21 September 2006) | doi:10.1038/nature05135; Received 21 June 2006; Accepted 24 July 2006; Published online 13 September 2006

Stoichiometry and turnover in single, functioning membrane protein complexes

Mark C. Leake1,3, Jennifer H. Chandler2,3, George H. Wadhams2, Fan Bai1, Richard M. Berry1 & Judith P. Armitage2

  1. Clarendon Laboratory, Department of Physics, University of Oxford, Parks Road, Oxford OX1 3PU, UK
  2. Microbiology Unit, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
  3. *These authors contributed equally to this work

Correspondence to: Judith P. Armitage2 Correspondence and requests for materials should be addressed to J.P.A. (Email: judith.armitage@bioch.ox.ac.uk).

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Many essential cellular processes are carried out by complex biological machines located in the cell membrane. The bacterial flagellar motor is a large membrane-spanning protein complex that functions as an ion-driven rotary motor to propel cells through liquid media1, 2, 3. Within the motor, MotB is a component of the stator that couples ion flow to torque generation and anchors the stator to the cell wall4, 5. Here we have investigated the protein stoichiometry, dynamics and turnover of MotB with single-molecule precision in functioning bacterial flagellar motors in Escherichia coli. We monitored motor function by rotation of a tethered cell body6, and simultaneously measured the number and dynamics of MotB molecules labelled with green fluorescent protein (GFP–MotB) in the motor by total internal reflection fluorescence microscopy. Counting fluorophores by the stepwise photobleaching of single GFP molecules showed that each motor contains approx22 copies of GFP–MotB, consistent with approx11 stators each containing two MotB molecules. We also observed a membrane pool of approx200 GFP–MotB molecules diffusing at approx0.008 microm2 s-1. Fluorescence recovery after photobleaching and fluorescence loss in photobleaching showed turnover of GFP–MotB between the membrane pool and motor with a rate constant of the order of 0.04 s-1: the dwell time of a given stator in the motor is only approx0.5 min. This is the first direct measurement of the number and rapid turnover of protein subunits within a functioning molecular machine.

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