Supplementary information

From the following article:

Editing-defective tRNA synthetase causes protein misfolding and neurodegeneration

Jeong Woong Lee, Kirk Beebe, Leslie A. Nangle, Jaeseon Jang, Chantal M. Longo-Guess, Susan A. Cook, Muriel T. Davisson, John P. Sundberg, Paul Schimmel and Susan L. Ackerman

Nature 443, 50-55(7 September 2006)

doi:10.1038/nature05096

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Supplemental Methods

This file contains additional details of the methods used in this study.

Supplementary Figure 1.

Pathway for acylated tRNAs entering the translational machinery.

Supplementary Figure 2.

Follicular dystrophy in sti/sti mutant mice.

Supplementary Figure 3.

The rotorod latency to fall in sti/sti and wild type mice.

Supplementary Figure 4.

Genes in the sti critical region.

Supplementary Figure 5.

The sti mutation is in the alanyl-tRNA synthetase (Aars) gene.

Supplementary Figure 6.

Secondary structure analysis of mutant and wild type human AlaRS.

Supplementary Figure 7

Normal aminoacylation of tRNAAla by A734E AlaRS.

Supplementary Figure 8.

Normal deacylation of Ala-tRNAAla by A734E AlaRS

Supplementary Figure 9.

Inherent misacylation with serine and glycine by mouse AlaRS

Supplementary Figure 10.

Accumulation of misfolded proteins in sti/sti Purkinje cells.

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