Supplementary information
From the following article:
The nitrate/proton antiporter AtCLCa mediates nitrate accumulation in plant vacuoles
A. De Angeli, D. Monachello, G. Ephritikhine, J. M. Frachisse, S. Thomine, F. Gambale and H. Barbier-Brygoo
Nature 442, 939-942(24 August 2006)
doi:10.1038/nature05013
Supplementary Figure 1
Malate currents: clca-2 knock-out mutant vacuoles show wild-type malate currents.
Supplementary Figure 2
AtCLCa current in different cytosolic NO3- concentrations. I-V curves of AtCLCa current in different cytosolic NO3- concentrations normalised to capacitance.
Supplementary Figure 3
Conserved regions of CLC proteins. Sequence alignment of conserved regions of different CLC proteins.
Supplementary Notes
Cytosolic solutions used for selectivity experiments in Figure 3c and d. Equation used for deriving the H+/NO3- transport. Numerical calculations.
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