FIGURE 2. MYPT-1 is the targeting subunit of merlin phosphatase.
From the following article:
Tumorigenic transformation by CPI-17 through inhibition of a merlin phosphatase
Hongchuan Jin, Tobias Sperka, Peter Herrlich and Helen Morrison
Nature 442, 576-579(3 August 2006)
doi:10.1038/nature04856
a, Co-immunoprecipitation (IP) of MYPT-1 and PP1
with merlin antibody (A-19). Immunoblots for MYPT-1 and PP1. b, Pull-down of purified recombinant C-merlin with GST–C-MYPT-1. c, Co-immunoprecipitations of wild-type and mutant merlin (S518A or S518D) with MYPT-1 from cell lysates. d, In vitro dephosphorylation of merlin by the MYPT-1–PP1 complex immunoprecipitated with anti-MYPT-1. e, Trypsinolysis of MYPT-1–PP1 strongly reduces the catalytic function (phospho-merlin added after trypsin inhibitor for the periods indicated).
