Supplementary information
From the following article:
Endothelial tubes assemble from intracellular vacuoles in vivo
Makoto Kamei, W. Brian Saunders, Kayla J. Bayless, Louis Dye, George E. Davis and Brant M. Weinstein
Nature 442, 453-456(27 July 2006)
doi:10.1038/nature04923
Supplementary Methods
Detailed descriptions of materials and methods used in this study.
Supplementary Movie Legends
Detailed legends for the Supplementary Movies.
Supplementary Movie 1
Time-lapse DIC imaging of cultured human umbilical vein endothelial cells forming, collapsing and fusing vacuoles.
Supplementary Movie 2
Time-lapse DIC imaging of a single cultured human umbilical vein endothelial cell forming vacuoles that merge and expand into a highly enlarged vacuolar space.
Supplementary Movie 3
Time-lapse 2-photon imaging of an embryonic zebrafish intersegmental vessel, with small vacuoles forming, collapsing and fusing.
Supplementary Movie 4
Time-lapse 2-photon imaging of an embryonic zebrafish intersegmental vessel forming an expanded vacuolar/lumenal space.
Supplementary Movie 5
Time-lapse 2-photon imaging of a lumenizing intersegmental vessel in a zebrafish embryo.
Supplementary Movie 6
Time-lapse DIC imaging of a lumenizing intersegmental vessel in a zebrafish embryo.
Supplementary Movie 7
Time-lapse DIC imaging of cultured human umbilical vein endothelial cells forming an intercellular space.
Supplementary Movie 8
Time-lapse DIC imaging of cultured human umbilical vein endothelial cells forming an intercellular space.
Supplementary Movie 9
Time-lapse epifluorescence imaging of cultured human umbilical vein endothelial cells forming an intercellular space without mixing of their respective cytoplasmic contents.
Supplementary Movie 10
Time-lapse 2-photon imaging of red quantum-dot-injected embryonic zebrafish trunk vessels.
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