FIGURE 1. GASC1 demethylates H3K9me3/me2.
From the following article:
The putative oncogene GASC1 demethylates tri- and dimethylated lysine 9 on histone H3
Paul A. C. Cloos, Jesper Christensen, Karl Agger, Alessio Maiolica, Juri Rappsilber, Torben Antal, Klaus H. Hansen and Kristian Helin
Nature 442, 307-311(20 July 2006)
doi:10.1038/nature04837
a, Fractions from size-exclusion chromatography of recombinant GASC1 were incubated with histones for 30 min at 37 °C and demethylation activity was assayed by immunoblotting. b, Demethylation activity in selected fractions assayed by immunoblotting. c, d, H3K9me3 peptides were incubated with or without GASC1 and the material was analysed by mass spectrometry (c) and immunoblotting (d). e, Demethylation assay using varying amounts of wild-type and mutant GASC1 on bulk histones. f, Predicted structure of GASC1 in complex with H3K9me3 tail (1–11) and its cofactors; the lysine H3K9 methyl groups are marked in yellow. Left panel shows an overview; right panel shows a magnification of the iron-binding residues. 
-KG, -ketoglutarate.
