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Letter

Nature 442, 192-194 (13 July 2006) | doi:10.1038/nature04810; Received 19 July 2005; Accepted 18 April 2006; Published online 14 May 2006

Isolation of a novel acidiphilic methanogen from an acidic peat bog

Suzanna L. Bräuer1, Hinsby Cadillo-Quiroz1, Erika Yashiro1,3, Joseph B. Yavitt2 & Stephen H. Zinder1

  1. Department of Microbiology and
  2. Department of Natural Resources, Cornell University, Ithaca, New York 14853, USA
  3. †Present address: Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706, USA

Correspondence to: Stephen H. Zinder1 Correspondence and requests for materials should be addressed to S.H.Z. (Email: shz1@cornell.edu).

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Acidic peatlands are among the largest natural sources of atmospheric methane and harbour a large diversity of methanogenic Archaea1. Despite the ubiquity of methanogens in these peatlands, indigenous methanogens capable of growth at acidic pH values have resisted culture and isolation2, 3, 4; these recalcitrant methanogens include members of an uncultured family-level clade in the Methanomicrobiales prevalent in many acidic peat bogs in the Northern Hemisphere1, 5, 6. However, we recently succeeded in obtaining a mixed enrichment culture of a member of this clade7. Here we describe its isolation and initial characterization. We demonstrate that the optimum pH for methanogenesis by this organism is lower than that of any previously described methanogen.