Supplementary information

From the following article:

Deregulation of a Ca2+/calmodulin-dependent kinase leads to spontaneous nodule development

Leïla Tirichine, Haruko Imaizumi-Anraku, Satoko Yoshida, Yasuhiro Murakami, Lene H. Madsen, Hiroki Miwa, Tomomi Nakagawa, Niels Sandal, Anita S. Albrektsen, Masayoshi Kawaguchi, Allan Downie, Shusei Sato, Satoshi Tabata, Hiroshi Kouchi, Martin Parniske, Shinji Kawasaki and Jens Stougaard

Nature 441, 1153-1156(29 June 2006)

doi:10.1038/nature04862

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Supplementary Figure 1

Calcium oscillations in root hairs in response to purified Nod factor.

Supplementary Figure 2

In vitro culture of wild type and snf1-1 mutant tissues.

Supplementary Figure 3

Alignment of chimeric Ca2+/calmodulin dependent protein kinases from Lotus, Medicago, pea, tobacco, lily, rice and Physcomitrella.

Supplementary Figure 4

The log-log plot corresponding to figure 4e.

Supplementary Methods

This file contains a detailed Methods section.

Supplementary Table 1

Summary of loss of function ccamk mutations and gain of function snf1 mutations.

Supplementary Table 2

This file shows complementation of ccamk-2 in the presence of Mesorhizobium loti; transformation of ccamk-2 in the absence of Mesorhizobium loti; and transformation of snf1-1 in the absence of Mesorhizobium loti.

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