Supplementary information
From the following article:
Nodulation independent of rhizobia induced by a calcium-activated kinase lacking autoinhibition
Cynthia Gleason, Shubho Chaudhuri, Tianbao Yang, Alfonso Muñoz, B. W. Poovaiah and Giles E. D. Oldroyd
Nature 441, 1149-1152(29 June 2006)
doi:10.1038/nature04812
Supplementary Methods
A complete list of the primers used to generate the DMI3 deletion constructs and a more detailed description of protocols.
Supplementary Table 1
ENOD11-GUS induction in dmi1, dmi2, nsp1, and nsp2 transformed with the DMI3 deletion constructs.
Supplementary Figure 1
Localisation of DMI3 and DMI3 deletion constructs fused to GFP (pdf 2.8 MB).
Supplementary Figure 2
Activation of ENOD11-GUS by DMI3-T271A and DMI3-T271D in dmi3-1 and DMI3 1-326 in dmi1 and nsp1 lines (pdf 556 KB).
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