FIGURE 3. Induction of ENOD11 by DMI3 gain-of-function constructs.
From the following article:
Nodulation independent of rhizobia induced by a calcium-activated kinase lacking autoinhibition
Cynthia Gleason, Shubho Chaudhuri, Tianbao Yang, Alfonso Muñoz, B. W. Poovaiah and Giles E. D. Oldroyd
Nature 441, 1149-1152(29 June 2006)
doi:10.1038/nature04812
a, b, Roots of dmi3-1 transformed with 35S-DMI3 show little GUS activity (a) but show activation of ENOD11-GUS when treated with 1 nM Nod factor (b). c, Cross-sections show an epidermal predominance of GUS staining. d, e, Roots transformed with 35S-DMI3 1–326 (d) and 35S-DMI3
328–355 (e) show ENOD11-GUS activity in the absence of Nod factor. f, Cross-sections of 35S-DMI3 1–326 indicate epidermal GUS expression. g, h, 35S-DMI3 1–326 can activate ENOD11-GUS in dmi2-1 mutants (g) but not in nsp2-1 mutants (h). Original magnification: a, b, d, e, 
10; c, f, 25; g, h, 15. i, RT–PCR shows that native ENOD11 is induced in root transformed with 35S-DMI3 1–326. Lane 1, dmi3-1 transformed with 35S-DMI3 treated with 1 nM Nod factor; lane 2, dmi3-1 transformed with 35S-DMI3; lane 3, dmi3-1 roots transformed with 35S-DMI3 1–326.
