FIGURE 5. Immunogenicity of hexon HVR-chimaeric rAd5 vectors in rhesus monkeys.
From the following article:
Hexon-chimaeric adenovirus serotype 5 vectors circumvent pre-existing anti-vector immunity
Diane M. Roberts, Anjali Nanda, Menzo J. E. Havenga, Peter Abbink, Diana M. Lynch, Bonnie A. Ewald, Jinyan Liu, Anna R. Thorner, Patricia E. Swanson, Darci A. Gorgone, Michelle A. Lifton, Angelique A. C. Lemckert, Lennart Holterman, Bing Chen, Athmanundh Dilraj, Angela Carville, Keith G. Mansfield, Jaap Goudsmit and Dan H. Barouch
Nature 441, 239-243(11 May 2006)
doi:10.1038/nature04721
Naive rhesus monkeys or rhesus monkeys with anti-Ad5 immunity were immunized intramuscularly with 1011 virus particles of rAd5-Gag (a, c) or rAd5HVR48(1–7)-Gag (b, d). a, b, Gag-specific cellular immune responses were assessed by IFN-
ELISPOT assays at multiple time points after immunization. c, d, Gag-specific CD4+ and CD8+ central memory (CM; CD28+CD95+) and effector memory (EM; CD28-CD95+) T lymphocyte responses were evaluated by pooled peptide IFN- ICS assays at week 4 after immunization.
