Supplementary information
From the following article:
A loss-of-function RNA interference screen for molecular targets in cancer
Vu N. Ngo, R. Eric Davis, Laurence Lamy, Xin Yu, Hong Zhao, Georg Lenz, Lloyd T. Lam, Sandeep Dave, Liming Yang, John Powell & Louis M. Staudt
Nature 441, 106-110(4 May 2006)
doi:10.1038/nature04687
Supplementary Figure 1
Knockdown of gene expression depends upon induction of shRNA expression by doxycycline. This figure shows Q-PCR and Western blot analysis of shRNA-mediated knockdown of target gene expression in DLBCL cell lines.
Supplementary Figure 2
Identification of shRNAs that block the proliferation or survival of lymphoma cell lines.
Supplementary Figure 3
Gene expression profiles and NF-
B pathway activity in DLBCL cell lines.
Supplementary Figure 4
CARD11 mRNA expression in ABC DLBCL, GCB DLBCL, and PMBL tumor biopsies.
Supplementary Table 1
Sequence of effective shRNAs and position within the targeted Refseq mRNA sequence.
Supplementary Methods
More detailed methods are described here for preparing doxycycline-inducible cell lines; performing barcode DNA microarrays; cell-based IKK assay; cytokine measurement; and survival assay.
Supplementary Figure Legends
Text to accompany the above Supplementary Figures.
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