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News and Views
Nature 440, 879-880 (13 April 2006) | doi:10.1038/440879a; Published online 12 April 2006
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Associate / Assistant Professor (Anatomy / Physiology) Department of Human Science
- Georgetown University, Department of Human Science
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Biological imaging: The diffraction barrier broken
Garth J. Simpson1
Abstract
The conventional optical limitations of fluorescence microscopy have been defied, to achieve nanoscale resolution of individual vesicle organelles at the junctions of neuronal cells.
Traditional optical microscopy cannot easily distinguish objects separated by less than about half the wavelength of visible light. For example, measurements of two fluorescent particles closer together than this 'diffraction barrier' generally produce one indistinct, bright blob.
- Garth J. Simpson is in the Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA.
Email: gsimpson@purdue.edu
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