Supplementary information

From the following article:

Deciphering the evolution and metabolism of an anammox bacterium from a community genome

Marc Strous, Eric Pelletier, Sophie Mangenot, Thomas Rattei, Angelika Lehner, Michael W. Taylor, Matthias Horn, Holger Daims, Delphine Bartol-Mavel, Patrick Wincker, Valérie Barbe, Nuria Fonknechten, David Vallenet, Béatrice Segurens, Chantal Schenowitz-Truong, Claudine Médigue, Astrid Collingro, Berend Snel, Bas E. Dutilh, Huub J. M. Op den Camp, Chris van der Drift, Irina Cirpus, Katinka T. van de Pas-Schoonen, Harry R. Harhangi, Laura van Niftrik, Markus Schmid, Jan Keltjens, Jack van de Vossenberg, Boran Kartal, Harald Meier, Dmitrij Frishman, Martijn A. Huynen, Hans-Werner Mewes, Jean Weissenbach, Mike S. M. Jetten, Michael Wagner and Denis Le Paslier

Nature 440, 790-794 (6 April 2006)

doi:10.1038/nature04647

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Supplementary Figure 1

Maximum-likelihood tree displaying the16S rRNA gene diversity in the anammox enrichment culture from which the K. stuttgartiensis genome was reconstructed.

Supplementary Figure 2

Representation of the K. stuttgartiensis chromosome including annotated coding sequences and RNA genes.

Supplementary Figure 3

Functional redundancy in catabolism and respiration in K. stuttgartiensis compared to other bacteria.

Supplementary Figure 4

Phylogenetic consensus tree based on concatenated 5S-16S-23S rRNA sequences, showing the phylogenetic positioning of K. stuttgartiensis within the Bacteria.

Supplementary Figure 5

Distance (FITCH with the Dayhoff-PAM model), maximum parsimony (MP) and maximum likelihood (PHYML with the Dayhoff-PAM model) phylogenetic trees based on 26 concatenated ribosomal protein sequences (see Supplementary Methods), showing the phylogenetic positioning of K. stuttgartiensis within the Bacteria.

Supplementary Table 1

General features of the Kuenenia stuttgartiensis genome and comparison with Rhodopirellula baltica.

Supplementary Table 2

Genes with known homologues involved in respiration.

Supplementary Table 3

Iron and manganese respiration by the anammox bacterium Kuenenia stuttgartiensis.

Supplementary Table 4

Genes with known homologues involved in carbon metabolism.

Supplementary Table 5

CO2 fixation pathway in Kuenenia stuttgartiensis.

Supplementary Table 6

Proteins (gene names) used for phylogenetic analysis.

Supplementary Table 7

List of orthologous groups (OG) in which both Chlamydiae and Planctomycetes are heavily represented, to the exclusion of most other sequenced bacteria.

Supplementary Table 8

Genes with homologues involved in peptidoglycan biosynthesis and cell division.

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