Supplementary information
From the following article:
A P-type ATPase required for rice blast disease and induction of host resistance
Martin J. Gilbert, Christopher R. Thornton, Gavin E. Wakley & Nicholas J. Talbot
Nature 440, 535-539(23 March 2006)
doi:10.1038/nature04567
Supplementary Figure 1.
Yeast complementation assay showing the Magnaporthe APT2 gene is unable to complement the cold sensitive phenotype of the Saccharomyces cerevisiae 
drs2 mutant.
Supplementary Figure 2.
Influence of MgAPT2(p) expression on aminophospholipid internalisation in intact yeast cells. MgAPT2(p) is unable to restore the ability of drs2
to internalize phosphatidylserine.
Supplementary Figure 3.
Generation and virulence of Mgapt2 mutants. Targeted gene replacement strategy showing the generation of 6 gene replacement transformants. Subsequent plant infection assays and epidermal penetration assays show a significant reduction of Mgapt2 mutants to cause rice blast disease.
Supplementary Figure 4.
Electron micrographs showing the accumulation of Berkeley bodies in the yeast drs2 mutant.
Supplementary Figure 5.
The loss of MgAPT2 has no affect on endocytosis. Wild type and Mgapt2 conidia were stained with FM4-64 and show no significant difference in uptake kinetics.
Supplementary Figure 6.
The Mgapt2 mutant is impaired in its ability to secrete 
-amylase. Using an anti--amylase antibody, immunogold experiments were conducted. The resulting electron micrographs show Mgapt2 is severely reduced in -amylase secretion.
Supplementary Figure 7.
Plate assay showing Mgapt2 mutants are not compromised in their ability to secrete the enzyme trehalase.
Supplementary Table 1.
Amino acid similarity and identity of MgAPT2 and the yeast aminophospholipid translocases.
Supplementary Table 2.
Table showing the ability of the Mgapt2 mutant to grow on single carbon sources.
Supplementary Table 3.
Summary of Magnaporthe strains used in the study.
Supplementary Table 4.
Summary of yeast strains used in the study.
Supplementary Methods.
Comprehensive and detailed explanation of protocols used in the study together with references.
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